Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Polarized Photometric Detector for High-Performance Liquid Chromatography
Atsushi YAMAMOTOAkinobu MATSUNAGAKazuichi HAYAKAWAEiichi MIZUKAMIMotoichi MIYAZAKI
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1991 年 7 巻 5 号 p. 719-721

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A photometric detector for an optically active compound is described in which two polarizers are set on both sides of the flow cell. When a nonchromophoric, optically active compound passes through the cell, the intensity of natural light transmitted through the polarizers and flow cell is changed. The detection of such a sample is possible photometrically. A large inclination angle between the polarizers increases the absorbance change and allows dextrorotatory and levorotatory to be differentiated. The direct HPLC/photometric detection of sugars is presented as an axample of this device.

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© The Japan Society for Analytical Chemistry
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