High-Performance Liquid Chromatographic Determination of Pyrroloquinoline Quinone as Acetone Adduct

Kenji KANO; Bunji UNO; Chie KAWASAKI; Kiyomi HORIKI; Satoshi KAWAI

doi:10.2116/analsci.7.737

Kenji KANO, Bunji UNO, Chie KAWASAKI, Kiyomi HORIKI, Satoshi KAWAI

著者情報

キーワード: Pyrroloquinoline quinone, high performance liquid chromatography, acetone adduct, ultraviolet detection

ジャーナルフリー

1991 年 7 巻 5 号 p. 737-739

DOI https://doi.org/10.2116/analsci.7.737

詳細

抄録

A high performance liquid chromatographic method for the determination of pyrroloquinoline quinone (PQQ) is described. PQQ reacts readily with acetone in a weakly alkaline medium, giving a stable adduct, 5-acetonyl PQQ. The adduct was separated on a Develosil C₁₈-5 column using a mixture of methanol and 0.06M phosphoric acid, and was detected at 254nm. The response was linear over the range (1-20)×10^-6M of PQQ and the detection limit was 2pmol for a 20-μl injection volume.

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