胃腺基底明調細胞のオートラジオグラフィーによる研究

抄録

The basal clear cell in the gastric gland of the rat and mouse was studied by means of autoradiography after injection of H³-dihydroxyphenylalanine (H³-DOPA), H³-5-hydroxytryptophan (H³-HTP), H³-tyrosine, C¹⁴-5-hydroxytryptamine and H³-tryptophan. The results were as follows.
1. After injection of H³-DOPA and H³-HTP, the characteristic strong radioactivity appeared in the basal clear cells as compared with the chief cells and parietal cells of the gastric glands.
2. The radioactivity in the clear cells was higher on the nucleus than the cytoplasm up to 15 minutes after the intraperitoneal injection of H³-DOPA or up to 30 minutes after the subcutaneous injection of H³-DOPA in contrast with the other amino acids used.
3. The radioactivity in the clear cells after the injection of H³-DOPA, decreased or vanished by the pretreatment, the simultaneous treatment and the posttreatment with α-methyldopa or reserpine.
4. The basal clear cells revealed some differences among them in the ability of incorporation of the amino acids.
6. Moderate radioactivity appeared on the argyrophile cells after the injection of H³-HTP.
From the results mentioned above, it is supposed that the basal clear cells, showing different phases of activity, might be the main sites of amine synthesis in the rat stomach, and more active than the enterochromaffine cells.