Protease-Catalyzed Peptide Synthesis in Ice

Sayed Mohiuddin Abdus Salam; Ken-ichi Kagawa; Katsuhiro Kawashiro

doi:10.11491/apcche.2004.0.819.0

Asian Pacific Confederation of Chemical Engineers congress program and abstracts

セッションID: 3P-01-098

DOI https://doi.org/10.11491/apcche.2004.0.819.0

会議情報

主催: The Society of Chemical Engineers, Japan

Protease-Catalyzed Peptide Synthesis in Ice

Sayed Mohiuddin Abdus Salam, Ken-ichi Kagawa, Katsuhiro Kawashiro

著者情報

キーワード: α-chymotrypsin, frozen aqueous system (ice), peptide synthesis, carbamoylmethyl (Cam) ester

会議録・要旨集フリー

詳細

抄録

The coupling efficiency in α-chymotrypsin-catalyzed peptide synthesis has been significantly improved and hydrolysis of an acyl donor comparatively decreased by the use of an N-benzyloxycarbonyl (Z)-protected amino acid carbamoylmethyl (Cam) ester as acyl donor in frozen aqueous solution. Under frozen state conditions, the protease was able to catalyze peptide bond formation more effectively than in aqueous reaction mixtures.

責任著者(Corresponding author)

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