Abstract
The antagonistic activities of ten strains of Trichoderma harzianum against eight strains of Lentinula edodes were investigated in a synthetic medium. In dual cultivation of T. harzianum and L. edodes, three types of hyphal interaction were observed, namely coiling of T. harzianum hypha around L. edodes hypha, growing of T. harzianum hypha in a wavy manner along L. edodes hypha and penetration of T. harzianum hypha into L. edodes hypha. The dominant hyphal interaction was hyphal coiling. In some fungal pairs more than one type of hyphal interaction were observed, while there were no hyphal interaction in other pairs. Volatile substances produced by two strains of T. harzianum exerted inhibitory activity on the mycelial growth of some L. edodes strains. Most of T. harzianum strains produced nonvolatile substances that inhibited the growth of various strains of L. edodes. However, the degrees of inhibition were different depending on virulence of T. harzianum strains and sensitivity of L. edodes.
