Abstract
Trichoderma causes, green mold, and inhibits the harvest of mushroom crops. We analyzed a total of 24 isolates of Trichoderma, Gliocladium and Hypocrea from mushroom facilities and on wild mushrooms, for molecular characteristics. We found random amplified polymorphic DNA (RAPD) markers to be useful for detecting clonal reproductions. A common fragment (A10-2000) was found in the T. harzianum aggregate isolates by RAPD analysis using primer A10. The single strand conformation polymorphisms (SSCP) analysis patterns of the internal transcribed spacer 1 (ITS1) region on the nuclear genome were characteristic of each species and aggregate.
