Abstract
The effect of using azuki bean-paste refuse as a substrate on the yield and constitution of fruit-bodies in the sawdust based cultivation of Flammulina velutipes (Curt.: Fr.) Sing, was investigated. By replacing the sawdust of Cryptomeria japonica (Lin. fil.) D. Don. (Sugi) with azuki bean-paste refuse, the fruit-body yield in the medium with a substitution ratio of more than 50% became constant and was 1.1 times the yield of the medium where only C. japonica sawdust was used. In the case where Picea jezoensis (Sieb.et Zucc.) Carr. (Yezo spruce) sawdust was replaced by azuki bean-paste refuse, the fruit-body yield increased as the substitution ratio of azuki bean-paste refuse became higher, up to a substitution ratio of 60%, where the fruit-body yield was 1.2 times greater compared to when only P. jezoensis sawdust was used. Though the fruit-body yield in the P. jezoensis (sawdust)・ricebran medium was less than that in the C. japonica (sawdust)・ricebran medium, the fruit-body yield in the medium where P. jezoensis sawdust was replaced by azuki bean-paste refuse at a ratio of 50〜80% was more than that of C. japonica (sawdust)・ricebran. These results suggest that P. jezoensis sawdust is useful as a substrate when mixed with azuki bean-paste refuse. By cultivating F. velutipes on the medium where P. jezoensis sawdust was replaced by azuki bean-paste refuse, the crude protein and crude fat content of the fruit-bodies decreased and the free sugar and free sugar alcohol, organic acid and free amino acid content increased. Especially, the content of arabinitol, glycerol, alanine and glycine in the fruit-bodies increased. Therefore, it was considered that the sweetness of the F. velutipes fruit-bodies was introduced by the increase in these substances.
