Abstract
Fistulina hepatica Schaeff. : Fr. has been known as a mushroom having antitumor and anti-bacterial properties. In order to obtain much mycelia, several kinds of the nutritional factors affecting mycelial growth of F. hepatica in a liquid culture were examined. Optimum initial pH of the medium was 5.0 and the mycelia had a remarkable character which is that the mycelia could not grow in neutral range from pH 6.5 to 7.0. Corn starch was most effective for growth in the ten kinds of carbon sources, which yielded over twice as much mycelia as glucose. This result suggests that the mycelia of F. hepatica has a superior capability to efficiently use highmolecular polysaccharides for its growth. The effects of nitrogen sources on growth were examined with ten kinds of organic and inorganic materials. Malt extract was the most effective for the growth, and the optimum concentration was 4.0%. In this study, we obtained the fundamental conditions for mass culture of F. hepatica mycelia.
