Abstract
The nutritional requirements for mycelial growth in Lyophyllum shimeji and L. fumosum were comparatively studied by using a liquid medium. The structure of vegetative mycelia in L. shimeji was morphologically different from that in L. fumosum, e. g., gelatinous, fine hyphae with thin cell wall in the former fungus, in contrast to rigid, thick hyphae with thick cell wall in the latter one. Glucose, trehalose, fructose and soluble starch were especially good carbon sources for mycelial growth in both species. Xylose and mannitol were poor carbon sources. Casamino acids, peptone, yeast extract, casitone and amino acids mixture were good nitrogen sources for the growth in L. shimeji. On the contrary, ammonium tartrate, ammonium chloride, ammonium nitrate and amino acids mixture were better nitrogen sources for mycelial growth than inorganic nitrogen sources in L. fumosum. Potassium nitrate did not support mycelial growth in both fungi. The most suitable C/N ratios in media for the growth of L. shimeji and L. fumosum were 40-90 and and 20-40, respectively.
