2002 年 50 巻 2 号 p. 235-236
In the present study, induction of maturation and ovulation and subsequent artificial insemination were tried in the cultured two-year-old brood stock of yellowtail, Seriola quinqueradiata to acquire fertilized eggs from young fish. After confirming the yolk accumulation by monitoring oocyte diameter through ovarian biopsy, fish with oocyte sizes between 700 and 740μm.....were selected. A single injection of HCG (500 IU/kg BW) could induce oocyte maturation and ovulation after 48 hours in all the fish except one. Enough volume (212, 000 eggs/fish) of floating eggs was obtained by the artificial insemination performed just after ovulation.