抄録
Soil microbial communities play a central role in the nitrogen cycle, and their function directly impacts crop nitrogen use efficiency. With the rapid advancement of multi-omics technologies such as metagenomics and metabolomics, the ability to reveal the composition, function, and dynamic changes of microbial communities has significantly improved. This study integrates metagenomics and metabolomics to systematically explore the effects of organic fertilizer application and water regulation on soil microbial community structure and function, and to elucidate their mechanisms in nitrogen cycle regulation. The results show that organic fertilizer significantly reshaped the microbial community composition and enhanced the expression of functional genes related to nitrogen transformation. Water regulation, on the other hand, further influenced nitrogen availability and mobility by modulating microbial metabolic activities. Based on large-scale integration analysis of multi-omics data, this study constructed a complex network between microbial communities and the nitrogen cycle, revealing the response patterns of microbes under different management practices and their contributions to nitrogen use efficiency. This study provides new insights into the complex relationship between soil microbes and nitrogen cycling, offering scientific support for optimizing agricultural management practices to improve nitrogen use efficiency.
