An Improved Method for Isolating Yeasts in the Genus Lipomyces and Related Genera from Soil

Takafumi NAGANUMA; Ken KATSUMATA; Tsutomu ANDO; Hisayuki WATANABE; Katsushi NISHIMURA; Yasuyuki UZUKA

doi:10.1271/bbb.63.195

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Microbiology & Fermentation Technology Notes

An Improved Method for Isolating Yeasts in the Genus Lipomyces and Related Genera from Soil

Takafumi NAGANUMA, Ken KATSUMATA, Tsutomu ANDO, Hisayuki WATANABE, Katsushi NISHIMURA, Yasuyuki UZUKA

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Keywords: soil yeast, Lipomyces yeast, cycloheximide, selective colony formation

JOURNAL FREE ACCESS

1999 Volume 63 Issue 1 Pages 195-198

DOI https://doi.org/10.1271/bbb.63.195

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Abstract

An improved method for isolating soil yeasts in the genus Lipomyces and related genera was developed on the basis of their physiological properties. Liberation of yeast cells from soil, capture of the yeast cells by a membrane filter, and colony formation on an acidic (pH 3) nitrogen-free agar plate containing 0.1% cycloheximide resulted in success.

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