New Method of Screening for Pressure-sensitive Mutants at High Hydrostatic Pressure

Noriaki MASUI; Chiaki KATO

doi:10.1271/bbb.63.235

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Microbiology & Fermentation Technology Notes

New Method of Screening for Pressure-sensitive Mutants at High Hydrostatic Pressure

Noriaki MASUI, Chiaki KATO

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Keywords: deep-sea, high hydrostatic pressure, barophilic bacteria, pressure-sensitive mutant, agar plate

JOURNAL FREE ACCESS

1999 Volume 63 Issue 1 Pages 235-237

DOI https://doi.org/10.1271/bbb.63.235

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Abstract

We have developed an efficient method of screening to detect pressure-sensitive mutants of barophilic or barotolerant bacteria using conventional agar medium plates. By this new method, 75 colonies can be screened per plate under high hydrostatic pressure.

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