Cloning and Expression in Escherichia coli of 2-Hydroxypropylphosphonic Acid Epoxidase from the Fosfomycin-producing Organism, Pseudomonas syringae PB-5123

Tomohisa KUZUYAMA; Takayuki SEKI; Seiji KOBAYASHI; Tomomi HIDAKA; Haruo SETO

doi:10.1271/bbb.63.2222

Biochemistry & Molecular Biology Notes

Cloning and Expression in Escherichia coli of 2-Hydroxypropylphosphonic Acid Epoxidase from the Fosfomycin-producing Organism, Pseudomonas syringae PB-5123

Tomohisa KUZUYAMA, Takayuki SEKI, Seiji KOBAYASHI, Tomomi HIDAKA, Haruo SETO

著者情報

キーワード: fosfomycin, biosynthesis, Pseudomonas syringae, 2-hydroxypropylphosphonic acid epoxidase

ジャーナルフリー

1999 年 63 巻 12 号 p. 2222-2224

DOI https://doi.org/10.1271/bbb.63.2222

詳細

抄録

The fosfomycin resistance gene, fosC, has been cloned from the fosfomycin-producing organism, Pseudomonas syringae PB-5123. Sequence analysis upstream of this gene found a new ORF showing significant homology to 2-hydroxypropylphosphonic acid epoxidase from fosfomycin-producing Streptomyces wedmorensis. The purified recombinant protein of this ORF converted 2-hydroxypropylphosphonic acid to fosfomycin. This result clearly showed the ORF to encode 2-hydroxypropylphosphonic acid epoxidase in PB-5123.

責任著者(Corresponding author)

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