Identification of Protein Kinase B (PKB) as a Phosphatidylinositol 3,4,5-Trisphosphate Binding Protein in Dictyostelium discoideum

Kenichi TANAKA; Hiroyuki ADACHI; Hiroaki KONISHI; Akihiro IWAMATSU; Katsuya OHKAWA; Toshiyuki SHIRAI; Satoshi NAGATA; Ushio KIKKAWA; Yasuhisa FUKUI

doi:10.1271/bbb.63.368

Biochemistry & Molecular Biology Regular Papers

Identification of Protein Kinase B (PKB) as a Phosphatidylinositol 3,4,5-Trisphosphate Binding Protein in Dictyostelium discoideum

Kenichi TANAKA, Hiroyuki ADACHI, Hiroaki KONISHI, Akihiro IWAMATSU, Katsuya OHKAWA, Toshiyuki SHIRAI, Satoshi NAGATA, Ushio KIKKAWA, Yasuhisa FUKUI

著者情報

キーワード: phosphatidylinositol 3,4,5-trisphosphate, PI-3 kinase, protein kinase B, Dictyostelium discoideum, phosphatidylinositol 3,4,5-trisphosphate binding protein

ジャーナルフリー

1999 年 63 巻 2 号 p. 368-372

DOI https://doi.org/10.1271/bbb.63.368

詳細

抄録

We have searched for phosphatidylinositol (PI)-3,4,5-trisphosphate (PIP₃) binding proteins in Dictyostelium discoideum using beads bearing a PIP₃ analogue, PIP₃-APB. One of the binding proteins with a molecular mass of 55 kDa was purified and its amino acid sequence was partially amalyzed. Database searches showed that the analyzed sequence was identical to that of protein kinase B (PKB) of D. discoideum. The specific activity of D. discoideum PKB, when expressed together with constitutively active PI-3 kinase in mammalian cells, was elevated by about three-fold, suggesting that PKB could also act downstream of PI-3 kinase in Dictyostelium cells.

責任著者(Corresponding author)

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