Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Increased Production of Lactonizing Lipase (LipL) from Pseudomonas sp. strain 109 by Lipids and Detergents
Junko TANAKATowa SUDOFumio IHARATakuya NIHIRAYasuhiro YAMADA
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1999 年 63 巻 5 号 p. 900-904

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  LipL of Pseudomonas sp. strain 109 is a unique lipase capable of catalyzing macrocyclic lactone synthesis using ω-hydroxyfatty acid esters as substrates. Several fatty acid esters were tested as inducers of LipL production. The addition of either soybean oil or a non-ionic detergent (Noigen HC) resulted in a 44 to 45-fold increase in extracellular LipL, and the presence of both resulted in a further 56-fold increase. Among the triglycerides tested, triolein was the most effective, with a 50-fold increase in LipL production. A Northern blot hybridization analysis found that the lipL transcript increased in the presence of soybean oil or Noigen HC, indicating that the production of LipL is regulated at the transcriptional level.

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© 1999 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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