Reidentification of Facultatively Alkaliphilic Bacillus sp. C-125 to Bacillus halodurans

Abstract

  Alkaliphilic Bacillus sp. C-125 was taxonomically characterized by physiological and biochemical characteristics, 16S rDNA sequence similarity, and DNA-DNA hybridization analyses. Phylogenetic analysis of strain C-125 based on comparison of 16S rDNA sequences showed that this strain is closely related to Bacillus halodurans. DNA-DNA hybridization analysis was done comparing C-125 and related Bacillus reference strains. The highest level of DNA-DNA relatedness (86%) was found between strain C-125 and B. halodurans. Our findings demonstrate that strain C-125 is a member of the species B. halodurans.