Carbohydrate-Dependent Hemolytic Activity of the Conjugate Composed of a C-Type Lectin, CEL-I, and an Amphiphilic α-Helical Peptide, 43-βAla2

Tomomitsu HATAKEYAMA; Tetsuji KAMINE; Yuko KONISHI; Hiromiki KUWAHARA; Takuro NIIDOME; Haruhiko AOYAGI

doi:10.1271/bbb.63.1312

Biochemistry & Molecular Biology Notes

Carbohydrate-Dependent Hemolytic Activity of the Conjugate Composed of a C-Type Lectin, CEL-I, and an Amphiphilic α-Helical Peptide, 4₃-βAla₂

Tomomitsu HATAKEYAMA, Tetsuji KAMINE, Yuko KONISHI, Hiromiki KUWAHARA, Takuro NIIDOME, Haruhiko AOYAGI

著者情報

キーワード: lectin, hemolysis, amphiphilic α-helical peptide, conjugation, carbohydrate-binding

ジャーナルフリー

1999 年 63 巻 7 号 p. 1312-1314

DOI https://doi.org/10.1271/bbb.63.1312

詳細

抄録

A lectin-cationic peptide conjugate, 4₃-CEL-I, was prepared from an invertebrate C-type lectin, CEL-I, and an amphiphilic α-helical peptide, 4₃-βAla₂ [Ac-(Leu-Ala-Arg-Leu)₃-βAla₂]. When 4₃-CEL-I was incubated with rabbit erythrocytes, hemolysis was observed, especially at basic pH. Inhibition experiment using some carbohydrates suggested that hemolytic activity of 4₃-CEL-I was caused by the interaction between 4₃-βAla₂ portion in the conjugate and the lipid bilayer after binding to the carbohydrate chains on the cell surface by the lectin activity of CEL-I.

責任著者(Corresponding author)

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