Efficient Production of Recombinant Human Erythropoietin by Replenishment of Microcarriers in the Hollow Fiber Culture Cassette

Yuichi INOUE; Nobuhisa ARITA; Kiichiro TERUYA; Yoshinori KATAKURA; Sanetaka SHIRAHATA

doi:10.1271/bbb.63.1624

Microbiology & Fermentation Technology Note

Efficient Production of Recombinant Human Erythropoietin by Replenishment of Microcarriers in the Hollow Fiber Culture Cassette

Yuichi INOUE, Nobuhisa ARITA, Kiichiro TERUYA, Yoshinori KATAKURA, Sanetaka SHIRAHATA

著者情報

キーワード: BHK-21 cells, hollow fiber bioreactor, microcarrier, human erythropoietin, ras oncogene

ジャーナルフリー

1999 年 63 巻 9 号 p. 1624-1626

DOI https://doi.org/10.1271/bbb.63.1624

詳細

抄録

Human erythropoietin (EPO)-producing recombinant BHK cells were cultured in culture medium containing microcarriers, and then microcarriers attached with cells were replenished in the hollow fiber culture cassette. By culture for 14 days, it was possible to produce 450 μg of the recombinant EPO, which corresponded to over two-fold of the recombinant EPO production by control hollow fiber culture without microcarriers.

責任著者(Corresponding author)

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