Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
A cDNA Encoding Ribosomal Protein RPL15 from the Desiccation-tolerant Bryophyte Tortula ruralis: mRNA Transcripts are Stably Maintained in Desiccated and Rehydrated Gametophytes
Qin ZENG, Andrew J. WOOD
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2000 年 64 巻 10 号 p. 2221-2224

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  A Tortula ruralis cDNA, Rpl15, encoding a predicted polypeptide with significant similarity to the large-subunit ribosomal protein L15 (RPL15) was isolated from a desiccated gametophyte cDNA library, and is the first L15 homologue identified from a bryophyte. The deduced 203 amino acid polypeptide is approximately 24.1 kDa, with a predicted pI of 11.1, and shares extensive identity with rat RPL15 deduced polypeptide (>57%). RNA blot hybridization using total RNA demonstrated that Rpl15 is constitutively expressed in moss gametophytes during a desiccation-rehydration cycle and that Rpl15 mRNA transcripts are maintained in desiccated gametophytes as conserved mRNAs.
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© 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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