Cloning and Sequencing of the Maltohexaose-producing Amylase Gene of Klebsiella pneumoniae

Abstract

  The molecular characterization of the maltohexaose-producing amylase gene of Klebsiella pneumoniae revealed an open reading frame in which 2,031 base pairs encode a protein of 677 amino acids with a calculated molecular weight of 75,921. The amylase gene had high similarities of 73.6% in DNA sequence and 79.3% in deduced amino acid sequence with the periplasmic α-amylase MalS gene of Escherichia coli.