Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene

Teisuke TAKITA; Naofumi SHIMIZU; Tokuo SUKATA; Satoshi HASHIMOTO; Eriko AKITA; Takuya YOKOTA; Nobuyoshi ESAKI; Kenji SODA; Kuniyo INOUYE; Ben’ichiro TONOMURA

doi:10.1271/bbb.64.432

Biochemistry & Molecular Biology Notes

Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene

Teisuke TAKITA, Naofumi SHIMIZU, Tokuo SUKATA, Satoshi HASHIMOTO, Eriko AKITA, Takuya YOKOTA, Nobuyoshi ESAKI, Kenji SODA, Kuniyo INOUYE, Ben’ichiro TONOMURA

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Keywords: Bacillus stearothermophilus, gene cloning, gene expression, lysyl-tRNA synthetase

JOURNAL FREE ACCESS

2000 Volume 64 Issue 2 Pages 432-437

DOI https://doi.org/10.1271/bbb.64.432

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Abstract

The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA1503 was cloned and sequenced. The gene consists of 1485 bp nucleotides commencing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids. The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promoter.

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