Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene

Teisuke TAKITA; Naofumi SHIMIZU; Tokuo SUKATA; Satoshi HASHIMOTO; Eriko AKITA; Takuya YOKOTA; Nobuyoshi ESAKI; Kenji SODA; Kuniyo INOUYE; Ben’ichiro TONOMURA

doi:10.1271/bbb.64.432

Biochemistry & Molecular Biology Notes

Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene

Teisuke TAKITA, Naofumi SHIMIZU, Tokuo SUKATA, Satoshi HASHIMOTO, Eriko AKITA, Takuya YOKOTA, Nobuyoshi ESAKI, Kenji SODA, Kuniyo INOUYE, Ben’ichiro TONOMURA

Author information

Teisuke TAKITA
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Naofumi SHIMIZU
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
Tokuo SUKATA
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
Satoshi HASHIMOTO
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
Eriko AKITA
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
Takuya YOKOTA
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
Nobuyoshi ESAKI
Institute for Chemical Research, Kyoto University
Kenji SODA
Institute for Chemical Research, Kyoto University
Kuniyo INOUYE
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Ben’ichiro TONOMURA
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

Keywords: Bacillus stearothermophilus, gene cloning, gene expression, lysyl-tRNA synthetase

JOURNAL FREE ACCESS

2000 Volume 64 Issue 2 Pages 432-437

DOI https://doi.org/10.1271/bbb.64.432

Details

Abstract

The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA1503 was cloned and sequenced. The gene consists of 1485 bp nucleotides commencing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids. The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promoter.

Corresponding author

Register with J-STAGE for free!