Purification and Some Properties of an Aminopeptidase from the Seeds of Cannabis sativa

Kazunari ARIMA; Tetsuya UCHIKOBA; Masayuki SHIMADA; Hiroo YONEZAWA; Makoto KANEDA

doi:10.1271/bbb.64.1055

Biochemistry & Molecular Biology Notes

Purification and Some Properties of an Aminopeptidase from the Seeds of Cannabis sativa

Kazunari ARIMA, Tetsuya UCHIKOBA, Masayuki SHIMADA, Hiroo YONEZAWA, Makoto KANEDA

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Keywords: Cannabis sativa, hemp seed, aminopeptidase, substrate specificity

JOURNAL FREE ACCESS

2000 Volume 64 Issue 5 Pages 1055-1057

DOI https://doi.org/10.1271/bbb.64.1055

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Abstract

An aminopeptidase (HSA) with a molecular mass of 78 kDa was purified from hemp (Cannabis sativa) seeds. The activity was inhibited by monoiodeacetic acid, p-chloromercuri-phenylsulfonic acid, and Zn²⁺ ion. The specificity of HSA was similar to that of a leucyl aminopeptidase [EC 3.4.11.1] from mammalian cytosol. However, other enzyme properties were different from these of leucyl aminopeptidase.

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