Application of a Single-cell Gel Electrophoresis (Comet) Assay to Screen the Antimutagenic Activity in Foods

Misao MIWA; Yasuhisa HONGO

doi:10.1271/bbb.64.1292

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Food & Nutrition Science Notes

Application of a Single-cell Gel Electrophoresis (Comet) Assay to Screen the Antimutagenic Activity in Foods

Misao MIWA, Yasuhisa HONGO

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Keywords: comet assay, DNA damage, antimutagen, meat offal, MeIQx

JOURNAL FREE ACCESS

2000 Volume 64 Issue 6 Pages 1292-1294

DOI https://doi.org/10.1271/bbb.64.1292

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Abstract

Three cell lines (HL60, U937 and RAW264.7) were studied for their sensitivity against mutagens by using a single-cell gel electrophoresis (comet) assay. RAW264.7, the most sensitive one, was chosen to screen the antimutagenic activity in swine and bovine offal. Aqueous extracts of the swine stomach (0.2 mg/ml) and heart (10 mg/ml) were found to have antimutagenic activity against MeIQx (+S9mix)-treated cells.

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