Unfolding of Poly-<small>L</small>-glutamic Acid by Microbubbling of Supercritical Carbon Dioxide

Hiroya ISHIKAWA; Mitsuya SHIMODA; Akiyoshi YONEKURA; Keiko MISHIMA; Kiyoshi MATSUMOTO; Yutaka OSAJIMA

doi:10.1271/bbb.64.1295

Biochemistry & Molecular Biology Notes

Unfolding of Poly-L-glutamic Acid by Microbubbling of Supercritical Carbon Dioxide

Hiroya ISHIKAWA, Mitsuya SHIMODA, Akiyoshi YONEKURA, Keiko MISHIMA, Kiyoshi MATSUMOTO, Yutaka OSAJIMA

著者情報

キーワード: poly-L-glutamic acid, supercritical CO₂, microbubbles, unfolding

ジャーナルフリー

2000 年 64 巻 6 号 p. 1295-1297

DOI https://doi.org/10.1271/bbb.64.1295

詳細

抄録

The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO₂ were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO₂. No important changes were observed in heating, autoclaving, or pH-lowering.

責任著者(Corresponding author)

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