Mutational Analysis of the Multicopy hao Gene Coding for Hydroxylamine Oxidoreductase in Nitrosomonas sp. Strain ENI-11

Akira YAMAGATA; Ryuichi HIROTA; Junichi KATO; Akio KURODA; Tsukasa IKEDA; Noboru TAKIGUCHI; Hisao OHTAKE

doi:10.1271/bbb.64.1754

Biochemistry & Molecular Biology Notes

Mutational Analysis of the Multicopy hao Gene Coding for Hydroxylamine Oxidoreductase in Nitrosomonas sp. Strain ENI-11

Akira YAMAGATA, Ryuichi HIROTA, Junichi KATO, Akio KURODA, Tsukasa IKEDA, Noboru TAKIGUCHI, Hisao OHTAKE

著者情報

キーワード: ammonia-oxidizing bacterium, gene disruption, hydroxylamine oxidoreductase, multicopy gene, Nitrosomonas

ジャーナルフリー

2000 年 64 巻 8 号 p. 1754-1757

DOI https://doi.org/10.1271/bbb.64.1754

詳細

抄録

The ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11 contains three copies of the hao gene (hao₁, hao₂, and hao₃) coding for hydroxylamine oxidoreductase (HAO). Three single mutants (hao₁::kan, hao₂::kan, or hao₃::kan) had 68 to 75% of the wild-type growth rate and 58 to 89% of the wild-type HAO activity when grown under the same conditions. A double mutant (hao₁::kan and hao₃::amp) also had 68% of the wild-type growth and 37% of the wild-type HAO activity.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）