Characterization of PBZ1, a Probenazole-inducible Gene, in Suspension-Cultured Rice Cells

Hideo NAKASHITA; Keiko YOSHIOKA; Masayuki TAKAYAMA; Rie KUGA; Naoki MIDOH; Ron USAMI; Koki HORIKOSHI; Katsuyoshi YONEYAMA; Isamu YAMAGUCHI

doi:10.1271/bbb.65.205

Biochemistry & Molecular Biology

Characterization of PBZ1, a Probenazole-inducible Gene, in Suspension-Cultured Rice Cells

Hideo NAKASHITA, Keiko YOSHIOKA, Masayuki TAKAYAMA, Rie KUGA, Naoki MIDOH, Ron USAMI, Koki HORIKOSHI, Katsuyoshi YONEYAMA, Isamu YAMAGUCHI

著者情報

キーワード: systemic acquired resistance, SAR, rice, probenazole

ジャーナルフリー

2001 年 65 巻 1 号 p. 205-208

DOI https://doi.org/10.1271/bbb.65.205

詳細

抄録

Probenazole (PBZ) induces non-race specific resistance in rice plants against rice blast fungus and PBZ1 was identified as a PBZ-inducible gene from rice. The induction of PBZ1 expression in suspension-cultured rice cells was investigated. Northern blot analysis indicated that PBZ1 was induced by PBZ in a dosedependent manner. Enzyme-linked immunosorbent assay (ELISA) showed a dose and time-dependent accumulation of PBZ1 protein. Both mRNA and protein analysis showed that PBZ1 was not induced by salicylic acid or an active metabolite, 1,2-benzisothiazole-1,1-dioxide.

責任著者(Corresponding author)

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