Inhibition of Ca2+-Signal-Dependent Growth Regulation by Radicicol in Budding Yeast

Ruthada CHANKLAN; Eiji AIHARA; Saori KOGA; Hidetoshi TAKAHASHI; Masaki MIZUNUMA; Tokichi MIYAKAWA

doi:10.1271/bbb.70502

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Inhibition of Ca²⁺-Signal-Dependent Growth Regulation by Radicicol in Budding Yeast

Ruthada CHANKLAN, Eiji AIHARA, Saori KOGA, Hidetoshi TAKAHASHI, Masaki MIZUNUMA, Tokichi MIYAKAWA

著者情報

キーワード: Ca²⁺ signaling, G₂ cell-cycle regulation, radicicol, Saccharomyces cerevisiae

ジャーナルフリー早期公開

論文ID: 70502

DOI https://doi.org/10.1271/bbb.70502

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 1 p.132

詳細

抄録

Compelled activation of Ca²⁺ signaling by exposure of zds1Δ strain Saccharomyces cerevisiae cells to external CaCl₂ leads to characteristic physiological consequences such as growth inhibition in the G₂ phase and polarized bud growth. Screening of microbial metabolites for activity alleviating the deleterious physiological effects of external CaCl₂ identified the Hsp90 inhibitor radicicol as an inhibitor of Ca²⁺-signal-dependent cell-cycle regulation in yeast. Radicicol alleviated analogous physiological effects due to the expression of a constitutively active form of calcineurin or overexpression of Swe1, the negative regulatory kinase of the Cdc28-Clb complex. Western blot analysis indicated that radicicol inhibited Ca²⁺-induced accumulation of Swe1 and Cln2.

責任著者(Corresponding author)

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