Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology
Green Marker for Colonies of Bacillus subtilis
Mitsuhiro ITAYASyed M. SHAHEDUZZAMANKuniko MATSUIAkira OMORITakashi TSUJI
Author information
JOURNAL FREE ACCESS

2001 Volume 65 Issue 3 Pages 579-583

Details
Abstract

A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.

Content from these authors

This article cannot obtain the latest cited-by information.

© 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Previous article Next article
feedback
Top