Reactivities of Mutants of a Major House Dust Mite Allergen Der f 2 to Mouse Anti-Der f 2 Monoclonal Antibodies Analyzed by Immunoblotting

Toshiro TAKAI; Midori AKAGAWA-CHIHARA; Toyokazu YOKOTA; Yasushi OKUMURA

doi:10.1271/bbb.65.694

Biochemistry & Molecular Biology

Reactivities of Mutants of a Major House Dust Mite Allergen Der f 2 to Mouse Anti-Der f 2 Monoclonal Antibodies Analyzed by Immunoblotting

Toshiro TAKAI, Midori AKAGAWA-CHIHARA, Toyokazu YOKOTA, Yasushi OKUMURA

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Keywords: major house dust mite allergen, Der f 2, site-directed mutagenesis, epitope, immunoblotting

JOURNAL FREE ACCESS

2001 Volume 65 Issue 3 Pages 694-697

DOI https://doi.org/10.1271/bbb.65.694

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Abstract

A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2, 7C10, 13A4, 15E11, and 18G8, to the recombinant Der f 2 variants were characterized by immunoblot analysis.

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