Novel Method Using Phytase for Separating Soybean β-Conglycinin and Glycinin

Abstract

A novel method for separating soybean β-conglycinin and glycinin from defatted soymilk by a phytase treatment was developed. Phytase was added to defatted soymilk (1000FYT/100 g of protein) at pH 6.0, and the miture incubated for 1 h at 40°C. This procedure separated β-conglycinin and glycinin without needing a reducing agent or cooling into the soluble and insoluble fractions, respectively. Simultaneously, most of the phytate in both proteins was removed.