Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Food & Nutrition Science
Novel Method Using Phytase for Separating Soybean β-Conglycinin and Glycinin
Tsutomu SAITOMitsutaka KOHNOKazunobu TSUMURAWataru KUGIMIYAMakoto KITO
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2001 年 65 巻 4 号 p. 884-887

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A novel method for separating soybean β-conglycinin and glycinin from defatted soymilk by a phytase treatment was developed. Phytase was added to defatted soymilk (1000FYT/100 g of protein) at pH 6.0, and the miture incubated for 1 h at 40°C. This procedure separated β-conglycinin and glycinin without needing a reducing agent or cooling into the soluble and insoluble fractions, respectively. Simultaneously, most of the phytate in both proteins was removed.

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© 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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