Use of 13C-labeled Glucose for Measuring Exogenous Glucose Oxidation in Mice

Kengo ISHIHARA; Shinichi OYAIZU; Wataru MIZUNOYA; Yoshiko FUKUCHI; Kyoden YASUMOTO; Tohru FUSHIKI

doi:10.1271/bbb.66.426

Food & Nutrition Science Notes

Use of ¹³C-labeled Glucose for Measuring Exogenous Glucose Oxidation in Mice

Kengo ISHIHARA, Shinichi OYAIZU, Wataru MIZUNOYA, Yoshiko FUKUCHI, Kyoden YASUMOTO, Tohru FUSHIKI

著者情報

キーワード: ¹³C, glucose, oxidation, mice

ジャーナルフリー

2002 年 66 巻 2 号 p. 426-429

DOI https://doi.org/10.1271/bbb.66.426

詳細

抄録

The author modified a respiratory gas analyzer to analyze the respiratory ¹³CO₂ of 12 small laboratory animals all at once. To investigate the practical use of this system, mice were orally (OR) or intravenously (IV) given glucose solutions containing three different amounts of ¹³C-labeled glucose. Expired ¹³CO₂ derived from exogenous glucose was detected within 10 minutes after administration in OR mice, but about 30 minutes in IV mice. The height of the peak of ¹³CO₂ expiration was correlated with the administered ¹³C-glucose mass.

責任著者(Corresponding author)

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