Spectroscopic Evidence That Salicylic Acid Converts a Temporally Inactivated Form of Horseradish Peroxidase (Compound III) to the Irreversibly Inactivated Verdohemoprotein (P-670)

Abstract

  We obtained spectroscopic evidence in support of salicylate-dependent inactivation of horseradish peroxidase-C. Addition of salicylate to the enzyme arrested at a temporal inactive state (Compound III) in the presence of H₂O₂, resulted in rapid and irreversible inactivation of the enzyme yielding verdohemoproteins (P-670). Multiple roles for salicylate in peroxidase- catalyzed reactions are discussed.