Continuous Cell-free Protein Synthesis Directed by Messenger DNA and Catalyzed by Extract of Thermus thermophilus HB27

Taketoshi UZAWA; Akihiko YAMAGISHI; Tairo OSHIMA

doi:10.1271/bbb.67.639

Biochemistry & Molecular Biology Notes

Continuous Cell-free Protein Synthesis Directed by Messenger DNA and Catalyzed by Extract of Thermus thermophilus HB27

Taketoshi UZAWA, Akihiko YAMAGISHI, Tairo OSHIMA

著者情報

キーワード: Thermus thermophilus, messenger DNA, cell-free protein synthesis, extreme thermophile, polyamine

ジャーナルフリー

2003 年 67 巻 3 号 p. 639-642

DOI https://doi.org/10.1271/bbb.67.639

詳細

抄録

Polypeptide synthesis directed by DNA as the messenger in a cell-free system of Thermus thermophilus was investigated. Polypeptides were synthesized with the addition of neomycin in the presence of DNA catalyzed by the cell extract. The stability of messenger DNA was greater than that of messenger RNA. Continuous cell-free translation with messenger DNA produced polypeptides at the rate of more than 8 μg/h in the presence of spermine.

責任著者(Corresponding author)

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