Preparation of Antiserum against Rat Δ6-Desaturase and Its Use to Evaluate the Desaturase Protein Levels in Rats Treated with Gemfibrozil, a Ligand for Peroxisome Proliferator-…

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  Anti-rat Δ6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat Δ6-desaturase. The antiserum prevented the enzymatic activity of Δ6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor α, is involved in activating Δ6-desaturase gene expression, thereby elevating the protein level and the activity.