Cloning and Overexpression of the avi2 Gene Encoding a Major Cellulase Produced by Humicola insolens FERM BP-5977

Tatsuki MORIYA; Manabu WATANABE; Naomi SUMIDA; Kaoru OKAKURA; Takeshi MURAKAMI

doi:10.1271/bbb.67.1434

Biochemistry & Molecular Biology Notes

Cloning and Overexpression of the avi2 Gene Encoding a Major Cellulase Produced by Humicola insolens FERM BP-5977

Tatsuki MORIYA, Manabu WATANABE, Naomi SUMIDA, Kaoru OKAKURA, Takeshi MURAKAMI

著者情報

キーワード: cellulase, Humicola insolens, avi2, protoplast

ジャーナルフリー

2003 年 67 巻 6 号 p. 1434-1437

DOI https://doi.org/10.1271/bbb.67.1434

詳細

抄録

The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.

責任著者(Corresponding author)

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