Nucleotide Sequences of Genes Encoding Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces

Hiroshi MATSUURA; Susumu OKAMOTO; Sarintip ANAMNART; Qiuqi WANG; Ze-Yang ZHOU; Takuya NIHIRA; Yasuhiro YAMADA; Tomohisa KUZUYAMA; Haruo SETO; Jiro NAKAYAMA; Akinori SUZUKI; Hiromichi NAGASAWA; Shohei SAKUDA

doi:10.1271/bbb.67.2002

Biochemistry & Molecular Biology Notes

Nucleotide Sequences of Genes Encoding Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces

Hiroshi MATSUURA, Susumu OKAMOTO, Sarintip ANAMNART, Qiuqi WANG, Ze-Yang ZHOU, Takuya NIHIRA, Yasuhiro YAMADA, Tomohisa KUZUYAMA, Haruo SETO, Jiro NAKAYAMA, Akinori SUZUKI, Hiromichi NAGASAWA, Shohei SAKUDA

Author information

Hiroshi MATSUURA
Department of Applied biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Susumu OKAMOTO
Department of Applied biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Sarintip ANAMNART
Department of Biotechnology, Osaka University
Qiuqi WANG
Department of Biotechnology, Osaka University
Ze-Yang ZHOU
Department of Biotechnology, Osaka University
Takuya NIHIRA
Department of Biotechnology, Osaka University
Yasuhiro YAMADA
Department of Biotechnology, Osaka University
Tomohisa KUZUYAMA
Institute of Molecular and Cellular Biosciences, The University of Tokyo
Haruo SETO
Institute of Molecular and Cellular Biosciences, The University of Tokyo
Jiro NAKAYAMA
Department of Applied biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Akinori SUZUKI
Department of Applied biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Hiromichi NAGASAWA
Department of Applied biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Shohei SAKUDA
Department of Applied biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Keywords: allosamidin, family 18 chitinase, family 19 chitinase, Streptomyces

JOURNAL FREE ACCESS

2003 Volume 67 Issue 9 Pages 2002-2005

DOI https://doi.org/10.1271/bbb.67.2002

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Abstract

Allosamidin is a strong inhibitor of family 18 chitinases. We previously reported the presence of allosamidin-sensitive and -insensitive chitinases (chitinase S and IS) in the culture filtrate of the allosamidin-producing strain, Streptomyces sp. AJ9463. In this study, we cloned and sequenced the genes encoding the two chitinases, which clarified that chitinase S and IS belong to the family 18 and 19 chitinase, respectively.

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