Construction of a Homologous Selectable Marker Gene for Lentinula edodes Transformation

Toshikazu IRIE; Toshitsugu SATO; Kumiko SAITO; Yoichi HONDA; Takashi WATANABE; Masaaki KUWAHARA; Hitoshi ENEI

doi:10.1271/bbb.67.2006

Biochemistry & Molecular Biology Notes

Construction of a Homologous Selectable Marker Gene for Lentinula edodes Transformation

Toshikazu IRIE, Toshitsugu SATO, Kumiko SAITO, Yoichi HONDA, Takashi WATANABE, Masaaki KUWAHARA, Hitoshi ENEI

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Keywords: Lentinula edodes, Pleurotus ostreatus, homobasidiomycetes, iron-sulfur protein (Ip) subunit

JOURNAL FREE ACCESS

2003 Volume 67 Issue 9 Pages 2006-2009

DOI https://doi.org/10.1271/bbb.67.2006

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Abstract

We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 μg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.

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