Molecular Characterization of a Rhodotorula-lytic Enzyme from Paecilomyces lilacinus Having β-1,3-Mannanase Activity

Hiroyuki SUGINO; Shinya FURUICHI; Sawao MURAO; Motoo ARAI; Takao FUJII

doi:10.1271/bbb.68.757

Biochemistry & Molecular Biology Notes

Molecular Characterization of a Rhodotorula-lytic Enzyme from Paecilomyces lilacinus Having β-1,3-Mannanase Activity

Hiroyuki SUGINO, Shinya FURUICHI, Sawao MURAO, Motoo ARAI, Takao FUJII

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Keywords: β-1,3-mannanase, glycoside hydrolase family 5, Paecilomyces

JOURNAL FREE ACCESS

2004 Volume 68 Issue 3 Pages 757-760

DOI https://doi.org/10.1271/bbb.68.757

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Abstract

We cloned the gene and corresponding cDNA for an extracellular Rhodotorula-lytic enzyme which has β-1,3-mannase activity, tentatively named MAN5C, from Paecilomyces lilacinus. MAN5C showed a high homology score with the members of glycoside hydrolase family 5 in a domain search with the Pfam database, indicating that MAN5C is a novel and unique member of glycoside hydrolase family 5.

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