The Role of the DRY Motif of Human MC4R for Receptor Activation

Yoshiaki YAMANO; Rio KAMON; Takao YOSHIMIZU; Yoshihisa TODA; Yuichi OSHIDA; Shigeyuki CHAKI; Masanobu YOSHIOKA; Isao MORISHIMA

doi:10.1271/bbb.68.1369

Biochemistry & Molecular Biology Notes

The Role of the DRY Motif of Human MC4R for Receptor Activation

Yoshiaki YAMANO, Rio KAMON, Takao YOSHIMIZU, Yoshihisa TODA, Yuichi OSHIDA, Shigeyuki CHAKI, Masanobu YOSHIOKA, Isao MORISHIMA

著者情報

キーワード: melanocortin type 4 receptor, site directed mutagenesis, cAMP, G-protein coupled receptor, DRY motif

ジャーナルフリー

2004 年 68 巻 6 号 p. 1369-1371

DOI https://doi.org/10.1271/bbb.68.1369

詳細

抄録

We constructed several mutant human MC4R cDNAs by site directed mutagenesis and expressed these receptors in COS-1 cells. The conserved DRY motif among GPCRs was mutated to generate eight mutants. While no MC4R ligand binding was detected in any of the mutants, one mutant, D146A, resulted in higher cAMP production in cells than the wild-type receptor without ligand stimulation.

責任著者(Corresponding author)

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