Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Promoting Protein, a Silkworm Hemolymph Protein Promoting in Vitro Replication of Nucleopolyhedrovirus, Binds to β-Glucans
Seiji MIYAKEYoshiaki YAMANOIsao MORISHIMA
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JOURNAL FREE ACCESS

2005 Volume 69 Issue 10 Pages 2012-2014

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Abstract

A protein which bound to 125I-labeled peptidoglycan (PGN) was isolated from hemolymph of silkworm larvae. The N-terminal amino acid sequence and the molecular weight of the protein were in accord with those described for Promoting Protein (PP) from the silkworm. The binding of the protein to [125I]PGN was competitively inhibited by various β-glucans. The binding kinetics of PGN and chitin to the protein were analyzed in a biosensor.

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© 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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