Promoting Protein, a Silkworm Hemolymph Protein Promoting in Vitro Replication of Nucleopolyhedrovirus, Binds to β-Glucans

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A protein which bound to ¹²⁵I-labeled peptidoglycan (PGN) was isolated from hemolymph of silkworm larvae. The N-terminal amino acid sequence and the molecular weight of the protein were in accord with those described for Promoting Protein (PP) from the silkworm. The binding of the protein to [¹²⁵I]PGN was competitively inhibited by various β-glucans. The binding kinetics of PGN and chitin to the protein were analyzed in a biosensor.