Purification and Characterization of Aldoxime Dehydratase of the Head Blight Fungus, Fusarium graminearum

Yasuo KATO; Yasuhisa ASANO

doi:10.1271/bbb.69.2254

Biochemistry & Molecular Biology Notes

Purification and Characterization of Aldoxime Dehydratase of the Head Blight Fungus, Fusarium graminearum

Yasuo KATO, Yasuhisa ASANO

著者情報

キーワード: aldoxime dehydratase, aldoxime-nitrile pathway, nitrilase, Fusarium graminearum, characterization

ジャーナルフリー

2005 年 69 巻 11 号 p. 2254-2257

DOI https://doi.org/10.1271/bbb.69.2254

詳細

抄録

Fungal aldoxime dehydratase (Oxd) of Fusarium graminearum MAFF305135 was purified and characterized for the first time from its overexpressing Escherichia coli transformant. The enzyme showed about 20% identity with known Oxds, and had similar enzymatic properties with nitrilase-linked Oxd from the Bacillus strain. It belongs to a group of phenylacetaldoxime dehydratases (EC 4.99.1.7), based on its substrate specificity and kinetic analysis.

責任著者(Corresponding author)

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