Production of Heterogeneous Dimer Lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli Cells

Shigehiro KAMODA; Tamami TERADA; Yoshimasa SABURI

doi:10.1271/bbb.69.635

Microbiology & Fermentation Technology Notes

Production of Heterogeneous Dimer Lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli Cells

Shigehiro KAMODA, Tamami TERADA, Yoshimasa SABURI

著者情報

キーワード: lignostilbenedioxygenase, ligninostilbene, stilbene, Sphingomonas, Pseudomonas

ジャーナルフリー

2005 年 69 巻 3 号 p. 635-637

DOI https://doi.org/10.1271/bbb.69.635

詳細

抄録

The lignostilbenedioxygenase isozyme I and III genes, lsdA and lsdB, were coexpressed within the same Escherichia coli cells. The lignostilbenedioxygenase isozymes produced were separated on QAE anion-exchange column chromatography. Three parts of active fractions corresponding to αα, αβ, and ββ dimers were detected. The purified isozyme from second active fractions corresponded to the native heterogeneous isozyme II.

責任著者(Corresponding author)

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