Molecular Monitoring of the Developmental Bacterial Community in the Gastrointestinal Tract of Japanese Infants

Prapa SONGJINDA; Jiro NAKAYAMA; Yumiko KUROKI; Shigemitsu TANAKA; Sanae FUKUDA; Chikako KIYOHARA; Tetsuro YAMAMOTO; Kunio IZUCHI; Taro SHIRAKAWA; Kenji SONOMOTO

doi:10.1271/bbb.69.638

Microbiology & Fermentation Technology Notes

Molecular Monitoring of the Developmental Bacterial Community in the Gastrointestinal Tract of Japanese Infants

Prapa SONGJINDA, Jiro NAKAYAMA, Yumiko KUROKI, Shigemitsu TANAKA, Sanae FUKUDA, Chikako KIYOHARA, Tetsuro YAMAMOTO, Kunio IZUCHI, Taro SHIRAKAWA, Kenji SONOMOTO

著者情報

Prapa SONGJINDA
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Jiro NAKAYAMA
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Yumiko KUROKI
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Shigemitsu TANAKA
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
Sanae FUKUDA
Department of Health Promotion and Human Behavior, Kyoto University Graduate School of Public Health
Chikako KIYOHARA
Department of Preventive Medicine, Division of Social Medicine, Graduate School of Medical Sciences, Kyushu University
Tetsuro YAMAMOTO
Department of Health Promotion and Human Behavior, Kyoto University Graduate School of Public Health
Total Technology Consultant
Kunio IZUCHI
Izuchi Hospital
Taro SHIRAKAWA
Department of Health Promotion and Human Behavior, Kyoto University Graduate School of Public Health
Kenji SONOMOTO
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University

キーワード: denaturing gradient gel electrophoresis (DGGE), gastrointestinal tract, infant, 16S rRNA, intestinal microbiota

ジャーナルフリー

2005 年 69 巻 3 号 p. 638-641

DOI https://doi.org/10.1271/bbb.69.638

詳細

抄録

The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then immediately replaced by facultative anaerobe, Enterococcus, Streptococcus, and Enterobacteriaceae through the first month, and finally strictly anaerobic Bifidobactrerium appeared.

責任著者(Corresponding author)

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