Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome: an Efficient Selection Method

Abstract

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.