Sequencing of Hemoglobin Gene 4 (dmhb4) and Southern Blot Analysis Provide Evidence of More Than Four Members of the Daphnia magna Globin Family

Frank NUNES; Désirée SPIERING; Marc WOLF; Alexandra WENDLER; Ralph PIROW; Rüdiger J. PAUL

doi:10.1271/bbb.69.1193

Biochemistry & Molecular Biology Notes

Sequencing of Hemoglobin Gene 4 (dmhb4) and Southern Blot Analysis Provide Evidence of More Than Four Members of the Daphnia magna Globin Family

Frank NUNES, Désirée SPIERING, Marc WOLF, Alexandra WENDLER, Ralph PIROW, Rüdiger J. PAUL

著者情報

キーワード: Daphnia magna, hemoglobin expression, hemoglobin genes, hemoglobin subunits

ジャーナルフリー

2005 年 69 巻 6 号 p. 1193-1197

DOI https://doi.org/10.1271/bbb.69.1193

詳細

抄録

A genomic fragment containing the hemoglobin gene dmhb4 of Daphnia magna was cloned and its nucleotide sequence determined. Concerning induction under hypoxic conditions, dmhb4 was found to be expressed constitutively with similar mRNA quantities in D. magna bred in either normoxic or hypoxic medium. Southern blot analysis revealed at least six hemoglobin-like sequences in the genome of Daphnia magna.

責任著者(Corresponding author)

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