Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Analytical Chemistry Note
Improvement of the Lectin-Antibody Enzyme Immunoassay of the Alphafetoprotein Carbohydrate Chain for Automation with the Enzyme Immunoassay Robot
Koichi TAMANOMika SUGIURAJun NATSUKIKazumi SAWAKAMI-KOBAYASHIHideji TAJIMAMasayuki MACHIDA
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2005 年 69 巻 8 号 p. 1616-1619

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The lectin-antibody enzyme immunoassay of the alphafetoprotein-L3 carbohydrate chain, a tumor marker of liver cancer, has not been automated. We improved the technique of the assay for automation. Consequently, alphafetoprotein-L3 and total alphafetoprotein were detected with two lectins using an automatic paramagnetic bead handling robot. This indicates that the improved method is potentially applicable to the automated enzyme immunoassay robot.
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© 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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